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KMID : 0914820060060030132
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Journal of the Korean Gastric Cancer Association
2006 Volume.6 No. 3 p.132 ~ p.138
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Roles of Dopamine in Proliferation of Gastric-Cancer Cells
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Jung Hee-Jun
Chae Hyun-Dong
Park Ki-Ho
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Abstract
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Purpose: Dopamine is a neurotransmitter, but in the GIT, the roles of dopamine are a regulator of epithelial cell proliferation, an endogenous protective factor, and a regulator of stomach cancer cell proliferation. By using two different gastric-cancer cell lines, we assessed the effects of dopamine and dopamine receptors on the proliferation of human gastric-cancer cells.
Materials and Methods: To assess the effects of dopamine and dopamine receptors on the proliferation of human gastric- cancer cells, we investigated cell proliferation and the expression of D1, D2L, and D2S receptor in two gastric-cancer cell lines, SNU 601 and KCU-C2. The effects of dopamine and dopamine receptors on the level of the cell proliferation were determined by staining with an A/H/E (acridine orange, hoechst and ethidium bromide) mixture.
Results: After dopamine treatment, the cell viability was significantly decreased in SNU 601 cells (P＜0.05) where the D2L receptor was absent, but not in KCU-C2 cells. After treatment with raclopride, a D2 receptor antagonist, dopamine-dose- dependent inhibition of cell proliferation was observed in SNU 601 cells (P＜0.05). After treatment with SCH 23390, a D1 receptor antagonist, dopamine significantly increased cell proliferation in KCU-C2 cells (P＜0.05), but inhibited cell proliferation in SNU 601 cells (no D2L receptor).
Conclusion: The dopamine signal via the D1 or the D2S receptor inhibited proliferation of gastric-cancer cells, but that via the D2L receptor increased proliferation. These results suggest that the regulatory effects of dopamine in the gastric-cancer cell proliferation may be controlled by using dopamine receptors. (J Korean Gastric Cancer Assoc 2006;6:132-138)
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KEYWORD
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Dopamine, Gastric cancer, Gastric-cancer cell line, Dopamine receptors
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